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transwell inserts corning costar 3422  (Corning Life Sciences)

 
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    Corning Life Sciences transwell inserts corning costar 3422
    Transwell Inserts Corning Costar 3422, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transwell+inserts+corning+costar+3422/96+well+plates/pm37469011-359-17-19
    Average 90 stars, based on 1 article reviews
    transwell inserts corning costar 3422 - by Bioz Stars, 2026-10
    90/100 stars

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    Migration:

    Article Title: The Oxysterol Receptor EBI2 Links Innate and Adaptive Immunity to Limit IFN Response and Systemic Lupus Erythematosus
    Article Snippet: Transwell inserts were removed from the 24 well plates (Corning costar, 3421), and migration media (600 μL) was added with recombinant human CXCL10 (100 ng mL −1 ) (Novoprotein, P02778) or conditional medium (the ratio of complete RPMI1640 medium and PIF of TMPD‐treated WT or Ebi2 ‐cKO mice was 2:1). .. For PEMs migration, PEMs (2 × 10 5 ) were seeded in the top of the Transwell inserts (Corning costar, 3422) in a complete DMEM, and conditional medium (the ratio of complete DMEM and PIF of TMPD‐treated WT or Ebi2 ‐cKO mice was 2:1) was added in the well below. .. The cell number in the receiving plates was recorded by Operetta (PerkinElmer).

    Article Title: The Oxysterol Receptor EBI2 Links Innate and Adaptive Immunity to Limit IFN Response and Systemic Lupus Erythematosus.
    Article Snippet: The cell number in the receiving plates was recorded by Operetta (PerkinElmer). .. PEMs Migration Assay: For PEMs migration, PEMs (2 × 105) were seeded in the top of the Transwell inserts (Corning costar, 3422) in a complete DMEM, and conditional medium (the ratio of complete DMEM and PIF of TMPD-treated WT or Ebi2-cKO mice was 2:1) was added in the well below. ..



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    KLF2 overexpression suppresses the metastasis of CRC cells. (A) Expression of KLF2 in HCoEpiC, FHC, SW480, and RKO cell lines was detected by western blot assay. (B) Western blotting assessment of KLF2 overexpression in SW480 and RKO cell lines. (C) The invasion activity of transfected SW480 and RKO cells was detected by transwell assay. Scale bar: 100 μm. (D) Statistical representation of the transwell assay shown in (C). (E) The migration activity of transfected SW480 cells was detected by wound healing assay. Scale bar: 100 μm. (F) The migration activity of transfected RKO cells was detected by wound healing assay. Scale bar: 100 μm. (G) Statistical representation of wound healing assays shown in (E) and (F). * P < 0.05; ** P < 0.01.

    Journal: The Journal of Pathology: Clinical Research

    Article Title: KLF2 inhibits colorectal cancer progression and metastasis by inducing ferroptosis via the PI3K / AKT signaling pathway

    doi: 10.1002/cjp2.325

    Figure Lengend Snippet: KLF2 overexpression suppresses the metastasis of CRC cells. (A) Expression of KLF2 in HCoEpiC, FHC, SW480, and RKO cell lines was detected by western blot assay. (B) Western blotting assessment of KLF2 overexpression in SW480 and RKO cell lines. (C) The invasion activity of transfected SW480 and RKO cells was detected by transwell assay. Scale bar: 100 μm. (D) Statistical representation of the transwell assay shown in (C). (E) The migration activity of transfected SW480 cells was detected by wound healing assay. Scale bar: 100 μm. (F) The migration activity of transfected RKO cells was detected by wound healing assay. Scale bar: 100 μm. (G) Statistical representation of wound healing assays shown in (E) and (F). * P < 0.05; ** P < 0.01.

    Article Snippet: Migration and invasion experiments were performed using Boyden chambers consisting of transwell membrane filter inserts (Corning Costar, cat. no. 3422, Life Sciences, New York, USA) covered in Matrigel.

    Techniques: Over Expression, Expressing, Western Blot, Activity Assay, Transfection, Transwell Assay, Migration, Wound Healing Assay

    Overexpression of KLF2 inhibits CRC metastasis by inducing ferroptosis via GPX4. (A) Transfected cells were treated with/without ferroptosis inhibitor Fer‐1 (2 μ m ) for 24 h and then GPX4 expression was detected by western blot assay. (B) The invasion activity of transfected SW480 and RKO cells was detected by transwell assay. Scale bar: 100 μm. (C) Statistical representation of transwell assay shown in (B). (D) The migration activity of transfected SW480 cells was detected by wound healing assay. Scale bar: 100 μm. (E) The migration activity of transfected RKO cells was detected by wound healing assay. Scale bar: 100 μm. (F) Statistical representation of transwell assay shown in (D) and (E). * P < 0.05; ** P < 0.01; *** P < 0.001.

    Journal: The Journal of Pathology: Clinical Research

    Article Title: KLF2 inhibits colorectal cancer progression and metastasis by inducing ferroptosis via the PI3K / AKT signaling pathway

    doi: 10.1002/cjp2.325

    Figure Lengend Snippet: Overexpression of KLF2 inhibits CRC metastasis by inducing ferroptosis via GPX4. (A) Transfected cells were treated with/without ferroptosis inhibitor Fer‐1 (2 μ m ) for 24 h and then GPX4 expression was detected by western blot assay. (B) The invasion activity of transfected SW480 and RKO cells was detected by transwell assay. Scale bar: 100 μm. (C) Statistical representation of transwell assay shown in (B). (D) The migration activity of transfected SW480 cells was detected by wound healing assay. Scale bar: 100 μm. (E) The migration activity of transfected RKO cells was detected by wound healing assay. Scale bar: 100 μm. (F) Statistical representation of transwell assay shown in (D) and (E). * P < 0.05; ** P < 0.01; *** P < 0.001.

    Article Snippet: Migration and invasion experiments were performed using Boyden chambers consisting of transwell membrane filter inserts (Corning Costar, cat. no. 3422, Life Sciences, New York, USA) covered in Matrigel.

    Techniques: Over Expression, Transfection, Expressing, Western Blot, Activity Assay, Transwell Assay, Migration, Wound Healing Assay